Author(s)

Zilin Hong

Corresponding Author

Zilin Hong

Abstract

Tetracycline is a common antibiotic, however, its residues are really harmful to both human health and the environment. To solve this problem, this study developed a bioremediation method based on synthetic biology and put it into use. The goal was to break down tetracycline in the environment, so it tried to make modified E. coli produce CotA laccase on their surface. Firstly, this paper used molecular cloning techniques to successfully make a recombinant plasmid. This plasmid can produce the pGAS-CotA fusion protein, and this project later confirmed the plasmid’s sequence was correct. After making sure the protein was being produced, this paper developed a tetracycline detection method based on ELISA, and it checked that this method worked. In the final degradation test, the bacterial strain the paper built showed a stable ability to break down low levels of tetracycline in the culture medium. This study shows a new possibility for using biology to clean up antibiotic pollution, and it also proves that synthetic biology could help solve environmental problems.

Keywords

Synthetic biology, tetracyline degradation, engineered E.coli, environmental pollution control, antibiotic resistance