Author(s)

Yuankang Sun

Corresponding Author

Yuankang Sun

Abstract

With the increase of offshore marine development activities, the pollution of phenolic compounds poses a serious threat to the offshore ecosystem. This paper focuses on the use of synthetic biology to solve the problem of phenol pollution in coastal areas. Firstly, the present situation of phenol pollution in coastal areas is analyzed, and it is clear that it has extensive sources, serious harm and insufficient public awareness. By screening Pseudomonas and other microorganisms, the genome of them was modified by CRISPR-Cas9 gene editing technology, and key enzyme genes were replaced and regulatory restriction genes were knocked out, so as to construct an efficient phenol degradation metabolic pathway. It was found that in this microbial pathway, the expression of key genes was dynamically regulated with the concentration of phenol, and phenol was gradually degraded into usable small molecules through a series of enzymatic reactions. This microbial approach based on synthetic biology design provides a new strategy for coastal phenol pollution control and is expected to improve the coastal ecological environment.

Keywords

Nearshore Phenol Pollution; Synthetic Biology; Microbial Pathway; Gene Expression Regulation; Enzymatic Reaction